Background: Fluoresceins labelled monoclonal antibodies against cell surface antigens are able directly to examine the expression of multiple antigens on the surface of lymphoid cells in Ulcerative colitis lesions.
Materials and methods: Using dual-immunofluorescence for investigated the coexpression of (CD3-CD19 and CD4-CD8) antigens on infiltrated lymphocytes of 47 patients with ulcerative colitis and 10 subjects from control group. Total and differential T cells as well as plasma cell counts were recorded in all tissue samples. Non parametric Kruskal-Wallis test was used to compare the median cell counts among different study groups.
Results: T lymphocyte, T-cell subtype and plasma cell in UC were high with highly statistical significant difference when compared with those in normal control subjects T cell UC (301.39) Controls (35.48), T-helper 2 UC (228.41) Controls (24.75), T- helper 1 UC (54.21) Controls (13.45) and plasma cell UC (45.46) Controls (12.94). Recorded values of were different and increased according to modified Geboes histological grades of disease activity T lymphocytes (r=0.944) followed by T helper 2 subtype (r=0.821), T helper 1 (r=0.653) and B lymphocyte (r=0.237).
Conclusions: The increased number of lymphocyte subsets was found in the UC lesions and correlated with different histological grades of disease activity.
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2009
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