Until now, the precise etiology of colorectal cancer (CRC) is not yet known. However, it is widely agreed that CRC develops slowly via a progressive accumulation of genetic mutations and involves inactivation of a variety of tumor- suppressor and DNA repair genes, with simultaneous activation of certain oncogenes due to long possible list of environmental factors. A total of 60 patients (22 were males and 38 were females) were involved in this study, they were divided according to histopathological diagnosis to three groups, colorectal cancer group, polyp group, and normal colonoscopy group. Samples from patients were selected between May 2012 and August 2014. From each patient enrolled in this study, 2 4 mucosal punch biopsies were taken for histopathology and immunohistochemistry and stool samples were taken and kept in RPMI (Rosewell Park Memorial Institute) medium, to be used in flow cytometry. Statistical analysis of the results showed that there was a significant difference in the positivity rate of S100A9 in histopathology sections and in the stool specimens of the three studied groups. The selected marker, S100A9 in histopathology specimens and in stool samples qualified as significant predictors or risk factors for having malignant CRC compared to healthy controls. While there was no significant difference regarding S100A9 expression as a risk factor between malignant tissues and non neoplastic polyp. And S100A9 in stool samples qualified as significant predictors or risk factors for having malignant CRC compared to healthy controls and non neoplastic polyp. In this study S100A9 showed to be a significant predictor for having CRC in both tissue and stool.
(FULL ARTICLE LINK) Read more ...
October 2015
|
|
|
