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Anti-angiogenic activity of Zizyphus spinachristi Leaves Extracts
Authors : Ahmed R Abu-Raghif, Hayder B Sahib, Muneer M Hanoon
The objective of the experiment is to investigate the possible anti-angiogenic activity of Zizyphus spinachristi leaves extracts. The leaves were dried and grounded into fine powder, and extracted successively with Petroleum ether, chloroform, methanol and water. Ex vivo rat aorta anti-angiogenesis assay was used to identify the most active anti-angiogenic extract. The most active extract has been chosen for dose response study. Free radical scavenging activity has been tested by 1,1 Diphenyl-2-picrylhydrazyl (DPPH) to detect which extract has the highest free radical scavenging activity to help determine the possible mechanism of action. Methanol extract was the most biologically active extract in terms of blood vessels growth inhibition in comparison with petroleum ether, chloroform and water extracts (P˂ 0.05). Methanol extract serial concentrations showed significant dose dependent inhibition activity (P<0.05) on rat aorta assay and IC50 was (29.08μg/ml). Methanol extract had the highest free radical scavenging activity in comparison with petroleum ether, chloroform and water extracts. The IC50 of DPPH scavenging activity for methanol extract was 33.91 μg/ml. The methanol extract of Zizyphus spinachristi leaves has potential anti-angiogenic activity and this activity may be due to the high free radical scavenging capacity.

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31-10-2015.