اختيار اللغة
وحدة البحوث الطبية العلاقات الثقافية شعبة ضمان الجودة و الأداء الجامعي وحدة التعليم الطبي و تطوير المناهج الدراسية لجنة التعضيد شعبة النشاطات الطلابية
  •  
  •  
  •  



Comparison of two virus concentration methods for enteric viruses detection in Moroccan wastewater and treated effluent
اروى مجاهد عبد الله
Authors : Hasna Amdiouni, Leena Maunula, Arwa Al-Shuwaikh, Jalal Nourlil.
Background: The effluents of wastewater treatment plants may contain infectious human viruses representing a major public health issue. Wastewater must be analysed for viruses detection using easy and rapid protocol. Objectives: To compare between two viral concentration methods used for detection of enteric viruses in wastewater and treated effluent. Then assess the presence of viral genomes by cell culture and PCR. Methods: Fifty samples of wastewater were collected from two wastewater treatment plants during one year survey (January-December 2009) in order to compare two virus concentration methods, the polyethylene glycol (PEG) precipitation and the two-phase separation method, advised by the World Health Organization guidelines. Then assess the presence of viral genomes of human enteroviruses (EV), human adenoviruses (HAdV), hepatitis A and E viruses (HAV and HEV), human noroviruses (HuNoV), human rotaviruses (RV) and human astroviruses (HAstV) by cell culture and polymerase chain reaction (PCR) technique. Results: This study, using three statistical tests, showed that there was no significant difference between the two concentration methods: the PEG precipitation and the two phase separation (P>0.05). Conclusion: Considering this study with protocol time, cost and simplicity. The PEG precipitation seems to be an alternative method of the two-phase separation method.

(FULL ARTICLE LINK) Read more ...
2017