Abstract : HSV-2 is thought to account the majority of cases of neonatal herpes,
which may cause severe complications in infected newborns.
Objective: This study was carried out to investigate the rate of HSV-2 infection in placental
tissue of women with spontaneous abortion by conventional polymerase chain reaction
technique (PCR).
Materials and methods: Placental tissue samples were collected from 100 pregnant women
with spontaneous abortion. Twenty five gram of the placental tissue was homogenized, the
homogenate centrifuged for about 15 min. at 5000 rpm and (2-8) °C. The supernatant used for
DNA extraction using DNA isolation kit ((DNA-sorb-B (Sacace)/ Italy) Kit).
Results: The results showed that, this gene was present in 19 (19%) out of 100 placental tissue
PCR product of this gene was 120 bp, the highest rate was observed in age group (30-39)
years. The highest fetal losses in studied group were occurred in the first trimester, there was a
statistical significant (P<0.006) association between number of pregnancy compared to
infection with HSV-2 and highly significant differences (P<0.001) with pregnancy outcome.
Conclusions: Herpes Simplex -2 Virus may play a significant role in pregnancy loss and can
be delivery outcome-related factor. Its detection by sensitive molecular techniques would
allow prompt therapeutic intervention in order to increase the possibility of a successful future
pregnancy.
Keywords : Herpes Simplex type 2, placental tissue, spontaneous abortion, first trimester,
gpG gene.
Introduction:
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15/5/2017 Vol.10 No.3, pp 545-551
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