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Genetic Variants in IL-10, IL-12B and IL-13 Genes and Susceptibility to Glioma
حيدر أحمد شمران
Authors : Haidar A. Shamran, Hayder F. Ghazi, Ahmed AL-Salman,
Background: Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. The exact causes of gliomas are not well illustrated; however, besides environmental risk factors large body of evidences indicated the involvement of genetic factors in the disease. Aims: Accumulating evidence suggests that inflammatory process play a role in glioma carcinogenesis. However, in this study, we explored whether polymorphisms inIL-10-1082A/G, IL-12p401188C/A, and IL-13+2044G/A with the incidence of gliomas in Iraqi patients. Subjects and Methods: A total of 96 gliomas patients with different grades, and 40 apparently healthy control individuals were recruited for this study. From each participant, blood sample was obtained from which genomic DNA was extracted. Amplification refractory mutation system and sequence specific primer PCR were used for genotyping of IL-10-1082A/G and IL-12p401188C/A respectively; whereas, IL-13+2044G/A was detected by DNA sequencing after the amplification of the gene using PCR and purification of products. Results: All SNPs were within Hardy-Weinberg equilibrium, and each of them appeared in three genotypes in patients and control. For IL-10-1082A/G, these genotypes were AA, AG and GG with frequencies of 75%, 22.93% and 2.07% respectively among patients, and 62.5%, 27.5% and 10% among control. The genotypes, For the variant IL-12p401188C/A, 72.92%, 23.96% and 3.13%% of patients had AA, AC and CC genotypes respectively compared to 65%, 30% and 5% respectively for control. Finally, the frequencies of IL-13+2044G/A genotypes (GG, GA, and AA) were 89.58%, 9.37% and 1.04% among patients versus 47.5%, 32.5% and 20% among control. Conclusion: The study suggests a protective role of mutant alleles (allele G and A) in IL-10-1082A/G and IL-13+2044G/A respectively against glioma. Future larger studies with more rigorous study designs are required to confirm the current findings.

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2014-12-1