Autophagy related 16 like 1 gene (ATG16L1) Thr300Ala genetic variant may be influence innate and adaptive immunity in Crohn’s disease. This study aims to determine the association tissue IL-17A with ATG16L1 T300A and clinical disease phenotype. This case control study involved 35 CD, 40 UC and 35 HC. After extraction of DNA from blood samples, ATG16L1 T300A genotyping were done by SSP-PCR. Direct immunofluorescence technique done for localization of lysozyme and IL-17A in tissue samples of all subjects. A highly significant increase in tissue IL-17A found in CD 36.4±7.89 and UC 30.23±5.86. Among CD, significant increase in IL-17A has been shown that CD risk allele carrier 37.97±6.96 compared with patients carrying normal allele 33.42±8.51. In other hand, IL-17A associated with inflammatory lesions 42.13±6.83 compared with stenosing 33.56±9.41 and penetrating 35.28±6.52. The study found that IL-17A associated with inflammatory lesions under the influence of ATG16L1 T300A genetic variant in CD.
(FULL ARTICLE LINK) Read more ...
1/2/2015
|
|
|
