Abstract
Back ground :The study of urinary excretion of some enzymes considered as a sensitive test for the detection of early stages of renal disease ,particularly N-acetyl-β-D-glucosaminidase (NAG) which is a hydrolytic lysosomal enzyme present in cells of the proximal convoluted tubule. Many clinical urological problems like (hydronephrosis ,vesicouretric reflux(VUR) and pyelonephritis ) carry a high risk , that require simple, fast and non-invasive marker .
Objectives: This study was performed to assess urinary NAG/ urinary creatinine (NAG/ Cr) ratio in patients with urological abnormalities (normal and abnormal ultrasonography) and compare it with normal healthy children.
Materials and Method: A prospective study was conducted from November, 2012 to April, 2013. Urine samples were collected from 70 patients with various urological abnormalities, and from 40 healthy control group, their age range (1month-13years). Children who were admitted to Emergency Pediatric department, Children Welfare Teaching Hospital / Medical City Complex, Baghdad, Iraq. The Glomerular filtration rate was in normal range in all of them. Urine samples were tested for NAG by (ELISA, Cusabio ,China) while, both Serum creatinine (S-Cr) and urine creatinine (U-Cr) were estimated by Jaffe’s kinetic method.
Results: In this study 28.5% of the patients had normal ultrasonography and 71.5 % abnormal ultrasonography .The current results revealed that NAG/creatinine index was significantly higher in all patients with (vesicoureteral reflux, hydronephrosis and pyelonephritis) in comparison with cystitis.
Conclusion: The assessment of urinary NAG could be considered as a useful marker in prediction of the (vesicoureteral reflux, hydronephrosis) .Urinary NAG is elevated in children with pyelonephritis and it can be considered as a further criterion in the diagnosis of upper urinary tract infection.
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2014 jun
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