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A Molecular approach to detecting type B Coxsackie virus in a cohort Iraqi patient with different type of abortion
ايناس عدنان عبد الرسول
Authors : Mervet B Jasim, Asmaa B Al-Obaidi, Enas Adnan Abdulrasul
Background The infection can present in newborns in different ways, ranging from no noticeable symptoms to more serious conditions like meningoencephalitis, myocarditis, and disseminated sepsis. Methods A cross-sectional study was conducted from November 2022 to June 2023. The study included 100 pregnant women with gestational ages ranging from 0 to 16 weeks, aged between 15 and 40 years. Each participant completed a standardized questionnaire. Afterward, placental tissue samples were collected from women who underwent elective termination of pregnancy. These samples were then analyzed for the presence of Coxsackievirus B (CVB) genome using a molecular biology approach. The detection process involved RNA extraction, cDNA, and qRT-PCR. Results A total of 100 women who had abortions were included in this study. Out of these, 88 had missed abortions, 10 had incomplete abortions …

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2024/4/12