The Department of Pharmacology at the College of Medicine, Al-Nahrain University, discussed the Ph.D. dissertation of the student Suhaib Mohammed Ibrahim, entitled: “The anti-angiogenic activity of Corynoline and Hordenine ex vivo in vivo and in vitro study.” This study aimed to evaluate and compare the anti-angiogenic effects of Corynoline and Hordenine. The objectives included assessing their efficacy in inhibiting microvessel formation, investigating their anti-proliferative effects on endothelial and cancer cells, and analyzing their interactions with key molecular targets involved in angiogenesis. Both Corynoline and Hordenine demonstrated a clear concentration-dependent inhibitory effect on angiogenesis in the RAR and CAM models, with complete inhibition of microvessel growth observed at concentrations ≥50 μg/ml and IC50 values of approximately 2585 μg/ml for each compound. In cellular studies, both compounds significantly reduced cell viability in a dose-dependent manner in endothelial and cancer cell lines. Corynoline showed slightly higher efficacy in suppressing the growth of HUVEC and MCF7 cells. Gene expression analyses revealed decreased VEGF expression in treated cancer cells, while molecular docking studies confirmed strong binding interactions with VEGFR2 and αvβ3, supporting a potential mechanism of angiogenesis inhibition. Corynoline and Hordenine thus exhibited prominent anti-angiogenic and anti-proliferative activities through inhibition of endothelial cell growth, suppression of the VEGF pathway, and high-affinity interactions with angiogenesis-related receptors. These findings highlight their therapeutic potential as natural anti-angiogenic agents in cancer treatment. Further in-depth studies are recommended to validate these results and clarify the underlying mechanisms. Examination Committee: Prof. Dr. Ahmed Rahma Abu-Rughayf – Chair Prof. Dr. Mohammed Jassim Hamza – Member Prof. Dr. Rafal Shakeeb Abdulwahab – Member Prof. Dr. Sarmad Hashem Kazem – Member Asst. Prof. Dr. Fouad Kazem Kati’ – Member Prof. Dr. Haider Baha Sahib – Member and Supervisor
The dissertation was accepted with an Excellent grade.
Division of Media and Government Communication
College of Medicine – Al-Nahrain University 
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