Salmonella is approved as a common foodborne pathogen, causing major health problems throughout
the world particularly in low‐ and middle‐income countries. Low-level fluoroquinolone resistance is conferred by
both chromosomal and plasmid-encoded resistance, this research was carried out look into the occurrence rate of
qnrA,qnrB and qnrS genes in Salmonella enterica serotype Typhi Cipr ofloxacin-resistant insulate from blood
samples of patients with typhoid fever. Fifteen Salmonella enterica serotype Typhi isolated previously from
patients with typhoid fever were included in this study. All bacterial isolates were confirmed to have ciprofloxacin
resistant by VITEK 2 microbial identification system; after plasmid DNA extraction; multiplex-PCR was done
with primer sequences intended to plasmid-mediated quinolone-resistance genes which is qnrA, qnrB, and qnrS.
In this study; it was 21 qur genes amongst 15 isolates. The qnrS gene was the commonest (10/21, 47.6%) followed
by qnrA (6/21, 28.5%), whereas only 4 isolates were positive for qnrB (5/21, 23.8%). Some isolates had more
than one qnr genes. So, Ciprofloxacin-resistant Salmonella typhi can have more than one gene at the same time;
and the most occurrence rate in regards to qnr gene in this study was qnrS compared to qnrA and qnrB
Keywords: Salmonella enterica serotype Typhi , Typhoid fever, Fluoroquinolone, Ciprofloxacin, qnr gene
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2022
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