This study aimed for molecular characterization of Acinetobacter baumannii isolated from patients with respiratory
tract infectionsfrom clinical specimens includes , sputum , lower respirayory secretion of patients admitted to
Medical Teaching City, and Al-Yarmouk Teaching Hospital in Baghdad, Al-Hussein teaching hospital in Karbala ,with
study the rate of biofilm formation in Acinetobacter baumannii, in addition to investigate theto determine the
frequency of responsible virulence genes. during a period from the first of October 2020 to the end of May
2021.The predominant symptom was Chest pain encountered in patients (22.45%). Ranking in the lowest order
Smoking is which was seen in patients (9.80%).In present study, out of 16 isolates there were (50%) possessed RecA
gene, Sur A gene (62.50%), PAI gene (56.20%) ,Fim H gene (81.20%) respectively.By PCR amplifiction, the present
results showed that, the amplification products of sequences of RecA gene, ITS gene ,Sur A gene PAI gene and Fim
H gene in chromosomal DNA were of size 425 bp 208bp,241bp,930bp and 508bp respectively .In the current study,
the distribution of genes in Acinetobacterbaumannii clinical isolates showed that, , the highly percentage gene Fim
H was identified in all isolates 20(80%),followed SurA 16(64%), ITS 14(56%) ,RecA Gene and PAI 12(48%)genes
respectively.
In Conclusion, In Iraqi hospitals, Acinetobacter baumannii was the most common bacterium found in patients with
respiratory illnesses.
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2021
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