Objective: To determine the possible genetic polymorphism rs10501087 genetic variants of BDNF gene and its relationship with major depressive disorder in Baghdad city.Method: Case control study, that the blood samples had taken from 120 participants were collected from 60 patients diagnosed as MDD according to DSM-V criteria by psychiatrist and 60 normal controls not suffer from any mood disorders and all participants had did not undergo any chronic disease. The collected blood divided into two samples, one for ELISA to estimate BDNF concentration and the other one to PCR for genotyping study.Results: Using statistical analysis for the evaluation the serum levels of BDNF and genetic variation, the levels of BDNF concentrations in depressed patients were estimated and there were significantly lower than healthy control group which was 729.12 pg.ml-1 in patients with depression compared with healthy control group which was 1169.5 pg.ml-1 p value (p<0.005).The results of HRM for rs10501087 in patients and healthy control groups showed that 14 (23%) of patients had wild type genotype (T/T), 13 (22%) of patients had the mutant variant (C/C), and 33 (55%) of patients had the heterozygous genotype (T/C). On the other hand, the screened controls, 26 (43%) were genotyped as T/T, 13 (22%) were genotyped as C/C and 21 (35%) were genotyped as T/C. Moreover, odd ratio for TT and CC genotypes in the studied groups, were 1.0 and 0.1857 respectively while for the heterozygous genotype it was 2.918, which motioned that T>C polymorphism was a risk factor for incidence of MDDConclusion: Brain Derived Neurotrophic Factor protein that estimated by ELISA method was decreasing significantly in depressive patients when compared with healthy control group. In other hand, there were significant differences (P≤0.05) between heterozygous T/C in BDNF gene SNP 10501087 variation in patients with MDD and healthy control groups P value (0.0123)
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6/11/2022
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