Nucleotide sequences of the Pseudomonas aeruginosa algD gene isolated from Iraqi patients with otitis media

Abstract

Out of 28 isolates of Pseudomonas aeruginosa from otitis media. The result revealed that the algD virulent gene was present in 21 isolates with 75 percentage. The gel electrophoresis showed that the molecular weight of algD gene was 1310 bp. DNA sequences of algD gene was done, and the results showed presence of some gene mutations like substitution, addition and deletion with 97% identity with the Refseq gene. From the other side, the results of identities of translated nucleotide sequence with the original sequence of amino acids revealed that there were effects of gene mutations on translation of the product protein.