Detection of Exo A Gene in Pseudomonas aeruginosa from Human and Dogs Using Polymerase Chain Reaction

Abstract

Pseudomonas aeruginosa is the most frequent etiological agent of otitis externa. In this study polymerase chain reaction was used to investigate the genotypic properties and genetic relationship between P. aeruginosa obtained from human and dogs with otitis externa in Iraq. Seventeen (17%)P. aeruginosawere isolated from 100 ear swabs of human and six isolate (6%) from 100 ear swabs of dogs by conventional methods (culture and biochemical tests). A total of 23 P. aeruginosa isolate from human and dog were subjected to PCR to detect the Exo A gene by amplifying a 396-bp region using the same primer. Results showed that, six(26 %) and two (8.6 %) isolates from human and dogs give positive product for exo A gene respectively. in conclusion, there is a genetic relationship between P. aeruginosa isolated from human and dogs in Iraq.