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ISOLATION AND MOLECULAR IDENTIFICATION OF ACINETOBACTER BAUMANNII IN COVID-19 PATIENTS
ثناء رشيد عبد الرحمن
Authors : Noor Flayyih Hasan1*, Thanaa Rasheed Abdulrahman2, and Jabbar R. Zangoor3
ABSTRACT : This study was conducted to investigate isolation and molecular identification of Acinetobacter baumannii in COVID-19 patients from clinical specimens includes, sputum, lower respirayory secretion of patients admitted to Medical Teaching City and Al-Yarmouk Teaching Hospital in Baghdad, Al-Hussein teaching hospital in Karbala, with study the rate of biofilm formation in Acinetobacter baumannii. In addition to investigate the to determine the frequency of responsible virulence genes. During a period from the first of October 2020 to the end of May 2021. A total of 100 respiratory patients 25 patients positive A. baumannii enrolled in this study, patient’s age ranged from (£ 25-88) year, 9 female and 16 male. A. baumannii were isolated and diagnosed primarily by characteristic features on culture media and biochemical tests then by VITEK 2 system to confirm diagnosis. A total of (25%) Acinetobacter baumannii were isolated in this study. The highest proportion (21%) of Acinetobacter baumannii was from sputum specimens, which include 27.59% in males and 21.43% in females, followed by (4%) in lower respiratory secretion of patients, which is all from males. According of infection a 25 A. baumannii isolated from covid-19 patients. the result showed that positive of A. baumannii 9 (36.0%) of isolated respiratory patients of Negative covid-19, while 16(64.0%) of positive A. baumannii of positive covid-19. In present study, out of 16 isolates of Covid 19 patient that were ITS gene (68.80%). In conclusion, high frequency of Acinetobacter baumannii infection was observed among (>45 year) and covid-19 patients.

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