Background Klebsiella pneumoniae (K. pneumoniae) is an important pathogen in hospital and community-acquired
urinary tract infections (HAUTI and CAUTI). It is showing a high resistance towards many antibiotics
Objective This study aims to estimate the percentage of K. pneumoniae in HAUTIs and CAUTIs, antibiotic
resistance, extended spectrum bata lactamase (ESBL), and detect the beta lactamase K.
pneumoniae carbapenemases (bla KPC) resistance gene and the presence of a mutation in this gene.
Methods A total of 200 urine samples were collected randomly; K. pneumoniae was cultured and isolated on
MacConkey agar, antibiotic sensitivity testing and ESBL detection were performed by Vitek 2
system, then DNA was extracted, polymerase chain reaction and sequencing for KPC gene were
done.
Results There was 7% (14 isolates) of K. pneumoniae (50%) HAUTI and (50%) CAUTI. ESBL was positive in 3
isolates (21.43%). The percentage of positive bla KPC gene was 42.8%. KPC gene sequencing showed
high rate of mutation in positive isolates reaching to 50 different nucleotides.
Conclusion This work has increased the knowledge on K. pneumoniae causing HAUTI and CAUTI in Iraqi
patients.
Keywords Klebsiella pneumoniae, hospital-acquired urinary tract infection, community-acquired urinary tract
infection, ESBL, bla KPC gene.
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2022/6
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