Flavipin, a metabolite produced by Aspergillus species is a potent antibiotic, antifungal,
anticancer, and nematocidal agent. The present study was conducted to investigate the cytotoxicity
and antimicrobial activity of flavipin produced by Aspergillus flavus. Eighty isolates of
Aspergillus species were screened for the presence of flavipin. Macroscopic, microscopic, and
molecular characterizations were performed to identify the Aspergillus species. Flavipin extract
was prepared, fractionated, and the concentration was determined in the selected isolates. The
antimicrobial activity of flavipin was tested against Candida albicans, Acinetobacter baumannii,
and methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration
(MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration
(MFC) were determined. A cytotoxicity test of flavipin was conducted on MCF-7 and WRL-68
cell lines. The results of the antibacterial activity of flavipin against MRSA and A. baumannii
showed a wide zone of inhibition of 35 and 30 mm, respectively. Flavipin exhibited the highest
antifungal activity against C. albicans, with inhibition zones of 44 mm in diameter. The MICs of
flavipin for both A. baumannii and MRSA were 31.2 μg/mL, while the MIC of flavipin for C.
albicans was 62.5 μg/mL. The MBC values of flavipin for MRSA and A. baumannii were 62.5
and 125 μg/mL, respectively, while the MFC of flavipin against C. albicans was 125 μg/mL. The
IC50 of flavipin was 52.34 μg/mL for the cancer cell line MCF-7 and 298.1 μg/mL for the normal
cell line WRL-68. The findings of the study revealed that flavipin produced by Aspergillus flavus
has antimicrobial and cytotoxic properties
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july, 2023
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