ABSTRACT
The study designed to investigate the nephroprotective effects of vitamin E and three different extracts of Origanum vulgare against
vancomycin-induced nephrotoxicity in rats. The Origanum vulgare were grounded to fine powder, and extracted sequentially with
chloroform, methanol and water. Forty eight male rats were divided randomly into six groups; and all groups were treated for 10
days. Group 1: control group received 5% dimethylsulphoxide (DMSO), Group 2: vancomycin group treated with (200 mg/kg/day,
IP), Group 3:Vitamin E group received (200mg/kg) orally, vancomycin (200mg/kg/ IP) was administered at fourth day and both were
continued till day 10, Group 3, 4 and 5 received Chloroform, methanol and water extract (CE) of Origanum vulgare respectively,
(100mg/kg) orally, for 10 days then vancomycin (200mg/kg/ IP) was administered at fourth day and both were continued till day 10.
Animals were sacrificed at day 11 and serum levels of creatinine, urea, albumin, total protein, Tumor Necrosis Factor-alpha (TNF-α)
as well as tissue levels of malondialdehyde were measured. Free radical scavenging activity was measured by DPPH assay. Vitamin E
and Origanum vulgare extracts produced significant reduction of serum levels of urea, creatinine, TNF-α and tissue MDA levels with
significant elevation in the serum levels of albumin and total protein when compared to vancomycin group. Methanol extract
showed significant effect on parameters above. Methanol extract had strong free radical scavenging activity with IC50 of (2.33μg/ml).
Origanum vulgare extracts and vitamin E showed significant nephroprotective activity.
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17/1/2016
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