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Activity of G6HD, ME, GPI and protease enzyme in Cryptosporidium oocyts isolated from Immunocompetent and Immunocompromised patients.
هدى ظاهر هذال
Authors :
Dr. Nada M. Al-Bashir1, Dr. Huda T. Al-Marsomy2, Dr. Jabbar Salman Hassan3, Ahlam Ismail 4 1, 2 and 3 Ph.D. Medical Microbiology/ College of medicine, Al-Nahrain University 4 M. Sc. Medical Microbiology Al-Kadhimyia Teaching Hospital jabbarsalman30@yahoo.com Abstract: Cryptosporidiosis is a clinical disease, usually presents as a gastro-enteritis-like syndrome, caused by infection with protozoan parasites of the Apicomplexan genus Cryptosporidium. Disease ranges in seriousness from mild to severe and signs and symptoms depend on the site of infection and nutritional and immune status of the host. This study was conducted to evaluate the activity of enzyme Glucose Phosphate isomerase (GPI), Malic Enzyme (ME), Glucose -6-Phosphate Dehdrogenase (G6PD) and Protease Enzyme in oocytes of Cryptosporidium species and if their significant difference between activity of oocytes isolation from immunocompetent patients or immunocompromised patients. Six hundred patients with an age range from 12 day to 18 years suffering from diarrhea. Modified Acid – fast stain (modified Ziehle-Neelsen) will be used in diagnosis of Cryptosporidium oocytes. Sixty six samples (11%) out of 600 patients were positive for Cryptosporidium oocytes by using modified Ziehle-Neelsen stain, regarding Enzyme activity results show highly activity of all enzymes included in this study with no difference in enzyme activity between Immunocompetent and immunocompromised patients. In this study we conclude that GPI, ME, G6PDH and Protease enzymes were found in Cryptosporidium Oocytes with highly activity, there was no significant difference between oocytes activity isolated from immunocompetent and immunocompromised patients.

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J Am Sci 2016;12(5):80-84