Background: Chronic hepatitis B virus (HBV) infection is a disease of worldwide distribution. IFN-γ is one of the key regulatory cytokine that influence the clearance of HBV. The production of this cytokine can be influenced by the presence of certain single nucleotide polymorphisms (SNPs) in IFN-γ gene. Subjects and Methods: This case-control study involved 62 chronic HBV outpatients and 40 apparently healthy individuals. Blood samples were collected from each participant, and DNA was extracted from each sample. IFN-γ gene was amplified by PCR technique using specific primers. The polymorphism in the gene of this cytokine was assessed by sequencing. Results: There was a significant difference in the frequency of AA genotype between patients (33.87%) and control (20%) (OR=3.341, 95%CI=1.075-10.387). Furthermore, allele A had higher frequency among patients (58.06%) compared with control (42.5%) with significant difference (OR= 1.873, 95%CI=1.06-3.309). Conclusion: allele A of the SNP+874A/T may be a predisposing risk factor for chronic HBV infection.
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19/1 / 2014
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