ABSTRACT—
Corresponding Author: 2*
Inflammasome activation cytokine were associated with bad seminal fluid analysis
parameters among infertile men with spinal cord injury suggesting pivotal role of NLRP3 pathway in male infertility. Objective: the present study aims to measurement of NLRP3 inflammasome mRNA expression and their activation cytokines (IL-1ß and IL-18) mRNA expression in semen samples in leukocytospermia and none leukocytospermia infertile men. Methods: semen specimen were collected from 264 infertile men; conventional semen analysis was done according to World Health Organization 2010. Total RNA was extracted from whole semen cells pellet then the relative mRNA expression of the NLRP3, IL-1ß and IL-18 in semen specimens was determined by using qRT- PCR. NLRP3 and caspase-3 proteins in both sperm and leukocytes were detected by Immunoperoxidase staining. Results: there was no significant differences in the amount of NLRP3, IL-1B and IL-18 mRNA expression and NLRP3 protein in the sperm cells between leukocytospermia and non-leukocytospermia infertile men (P>0.05). In contrary there was high significant difference in the NLRP3 protein in leukocytes between these two groups (p≤0.001). While, translated protein of caspase-3 in both sperm and leukocytes was no significantly differed between these two groups (P>0.05). Conclusion: Current study suggests that the NLRP3, IL-1ß were expressed in cellular components of semen sample and their expression unrelated to the state of leukocytospermia, while po-IL- 18 is constitutively expressed in leukocytes, epithelial cells and defective sperm cells and released from these
cells into seminal fluid after undergoing to apoptosis and necrosis pathways.
(FULL ARTICLE LINK) Read more ...
1-8-2020
|
|
|
