مي فاضل ماجد
Authors : Zahra Ismail Abdel-Hussein
Wound healing is an extremely common and vital process coordinately mediated by multiple cell types and molecular pathways. Basic fibroblast growth factor (bFGF) is one of major growth factors thatare related to the healing processinjured skin. The current study aimed to evaluate the effects of this growth factor and it’s direct expression changein healing process and to detect key role phase. This study was conducted on forty adult male albino mice aged between 3-6 months, with 25-28 ± 50 g body weight, these were divided into five groups(8 animals in each ), namely the experimental and control groups. Experimental groups had linear incision of the skin about 2-3 cm in length on the back, injury was followed in 4 different times, after one day, 3day, one week and 2week. In addition to control group (without injury) all animals were treated according to National Institute of Health (NIH). The skin tissues were collected after injury and processed for paraffin blocks. Paraffin section of 5ìm thickness was made, for general histological examination hematoxyline and eosin was used. The tissue sections were treated with anti-FGF2 (sc-74412). The Immunohistochemical changes were quantifiable experimental groups and the control using Aperio Scope Image analysis software that was used to evaluate the Immunohistochemical reactivity. The statistical analysis using SPSS software was performed. Histological examination of H&E stained section were meant to follow up healing process. The site of injury after clot formation showed heavy infiltration of different types of inflammatory cells. The mean level of bFGF decrease in the second group first day after injury (FD) (0.609 pixl/ì²) in comparison to first group (control) (0.675 pixl/ì²), moreover, the third group expose redaction in the number of inflammatory cells and thickness edges of epidermis adjacent to the site of injury with approaching of both dermal and epidermal line of demarcation, while the mean level increased in the third group third day after injury (RD) (0.769 pixl/ì²) to reached high level comparison with another groups. The main changes in the fourth group in the first week after injury (1wk) showed heavy vascular infiltration of the dermal area with few number of inflammatory cells was seen scattered near dermal area in the site of injury, large number of fibroblasts and endothelial cells in the dermal layer at the wound bed and we found new collagen fibers in the stage. In the some experimental animals in this group we showed a completed thin epidermal layer (early healing), while others in same group were (delayed healing) according to immunity of animals. From this study, it has been concluded that healing progression is a process with overlapping phases and that bFGF is significantly elevated in the 1st week and starts to decline by the end of 2rd week. This timing can be used as a guide line in using topical and injection of bFGF after injury to enhance healing process.

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