Background Nephrotoxicity is one of the most important side effects and therapeutical limitations of aminoglycoside antibiotics. Nephrotoxicity appears in 10–25% of therapeutic courses. Amikacin nephrotoxicity has been considered to result mainly from tubular damage. Vardenafil is a selective and potent inhibitor of specific phosphodiesterase 5 enzyme. It is an oral therapy for the treatment of erectile dysfunction. Aim of the study To study the nephroprotective effects of vardenafil against amikacin induced nephrotoxicity in rabbits. Materials and methods Twenty four domestic male rabbits were randomly divided into three groups , each of eight animals as follow: Group1:(control group) treated with dimethylsulfoxide (DMSO) orally. Group 2: (amikacin group) or (induction group) treated with daily dose of (100 mg/kg/IM ) for 21 days. Group 3: (vardenafil group) which treated with daily oral dose of (2 mg/kg) and given amikacin (100mg/kg/ IM) at fourth day and both continued till day 24. Animals were sacrificed at day 25. Changes in serum levels of creatinine, urea, albumin, total protein, tissue malondialdehyde (MDA) and histopathological scores of all groups were measured. Results Serum levels of creatinine, urea, albumin, total protein, tissue MDA and histopathological scores analysis were done at day 25 after induction of nephrotoxicity, showed that vardenafil caused significant reduction of serum levels of creatinine, urea, tissue MDA and histopathological scores (p<0.05) in comparison to amikacin group. Serum levels of albumin and total protein , vardenafil caused significant elevation of serum levels of albumin and total protein (p<0.05) in comparison to amikacin group . Conclusion Vardenafil was exerted nephroprotective effect through correction the investigation parameters of this study that had been changed due to amikacin.
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November 2014
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