ABSTRACT
Objectives: The aim of this study was to investigate the capability of
Vitex agnus-castus methanol extract which showed anti-angiogenic
activity by Hayder and co-worker in previous study, to inhibit cancer
cell line proliferation, and to identify the possible mechanism of
action.
Methods: The anti-proliferative activity of the methanol extract
tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium
bromide (MTT) assay on breast cancer cell line (MCF7) and Prostate
cancer cell line (PC3) which were purchased from American type
culture cell (ATCC). 1, 1-diphenyl -2-picrylhydrazyl (DPPH assay)
has been used to identify the free radical scavenging activity.
Results: Dose response relationship was shown. No significant
cytotoxic activity of methanol extract has identified on MCF7 and
PC3, the IC50 were 46.52μg/ml and 90.01μg/ml respectively.
Methanol extract presented free radical scavenging capability the
IC50 was (126.79mg/ml).
Conclusion: These finding showed that methanol extract had a
significant dose-dependent efficiency against the growth of the cells
MCF7 and PC3. At the same time, the extract did not show any
cytotoxic action at the applied dose, so no toxic effect against the
MCF7 and PC3 cell line can be expected in vitro. The capability of
the Vitex agnus-castus methanol extract in scavenging the free
radicals may elucidate the mechanism of action on cell line.
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12/2/2015
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