Dipolma in Biochemistry

1st Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Biochemistry	CHMBch-D1	2		2
2	Biostatistics	COMSta-D1	2		2
3	Clinical chemistry	CHMCch-D1	2	1	3
4	English language	ENGEng-D1	1		1
5	Lab. Technique	CHMLtc-D1	2	1	3
6	Seminar	CHMSem-D1	1		1
Total					14

2nd Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Basic immunology	CHMBim-D2	1		1
2	Clinical chemistry	CHMCch-D2	2	1	3
3	Lab. Techniques	CHMLtc-D2	2	1	3
4	Selected topics in biochemistry	CHMSto-D2	2		2
5	Seminar	CHMSem-D2	1		1
Total					10

Biochemistry MSc Curriculum

1st Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Biochemistry	CHMBio-M1	3	-	3
2	Biostatistics	COMSta-M1	2	-	2
3	English language	ENGEng-M2	1	-	1
4	General Chemistry	CHMGen-M1	2	-	2
5	Histochemistry	CHMHch-M1	1	-	1
6	Lab. techniques	CHMLtc-M1	2	3	3
7	Seminar	CHMSem-M1	1	-	1
Total					13

1. Biochemistry (3 credits)

Theory: 3 hours/ Week

TOPICS

1. Bioenergetics     (3 hours)
2. Biological membranes and transport mechanisms      (6 hours)
3. Carbohydrates: structure, function, metabolism         (9 hours)
4. Lipids: structure, function, metabolism                      (9 hours)
5. Amino acid and protein metabolism(9 hours)
6. Creatine and biogenic amine metabolism                   (4 hours)
7. Enzymes: nomenclature and classification                 (5 hours)

1. General Chemistry (Two credits)

Theory: 3 hours/week

TOPICS
    

1. Rate of reaction, the order of reaction half-life of reaction.
2. Kinetics of multi-step reactions, rate constant of reversible reactions.
3. Temperature effect on the velocity of a reaction, activation energy, Catalysis.
4. Acid-base catalysis, heterogeneous catalysis.
5. Enzymes catalytic efficiency, specificity.
6. Chemical equilibrium, equilibrium constant, factors affecting the chemical equilibrium, Le Chatelier's principle.
7. Mechanism of enzyme action, Vmax, effect of temp., effect of pH.
8. Michaelis-Menten equation, effect of substrate concentration on reaction velocity, Lineweaver- Burk plot.
9. Inhibition of enzyme activity.
10. Adsorption, types of adsorption, adsorption isotherms.
11. Langmuir adsorption, types of isotherms adsorption of solutes by solids, adsorption from solution.
12. Wetting agents, Retarded reactions, heterogeneous reactions, catalytic poisons.

1. Laboratory techniques (Three credits)

Theory: 2 hours/week
Practical: 3 hours/week

TOPICS

1. Specimen collection and quality control                  (4 hours)
2. Photometry: Spectro-, Flouro-, flame- and atomic absorption           (6 hours)
3. Chromatography: Gel filtration and ion-exchange  (4 hours)
4. Electrochemistry                (4 hours)
5. Electrophoresis technique  (2 hours)
6. Radio-immunoassay and ELISA technique            (4 hours)
7. Centrifugation and ultra- centrifugation                  (3 hours)
8. Tissue homogenization       (2 hours)

Biochemistry MSc Curriculum

2nd Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Basic immunology	CHMBim-M2	2	-	2
2	Clinical chemistry	CHMCch-M2	2	3	3
3	Endocrinology	CHMEnd-M2	1	-	1
4	Nutrition	CHMNut-M2	1	-	1
5	Research Methodology	COMRme-M2			1
6	Selective topics	CHMSto-M2	2	-	2
7	Seminar	CHMSem-M2	1	-	1
Total					11

1. Clinical Chemistry (3 credits)

Theory: 2 hours/week
Practical: 3 hours/week

TOPICS

1. Carbohydrate disorders       (4 hours)
2. Lipid disorders   (2 hours)
3. Amino acids and protein disorders                 (4 hours)
4. Liver function tests              (2 hours)
5. Renal function tests             (2 hours)
6. Water and electrolyte regulation                     (6 hours)
7. Clinical enzymology            (4 hours)
8. Extreme age chemistry         (4 hours)

1. Endocrinology (2 credits)

Theory: 2 hours/week

TOPICS

1. Definition, classification and chemistry                     (2 hours)
2. Synthesis    (2 hours)
3. Mode of action  (2 hours)
4. Hormone action and signal transduction                    (4 hours)
5. Hormone receptors                           (2 hours)
6. Target cell concept                           (2 hours)
7. Hypothalamus and pituitary hormones                       (4 hours)
8. Control and regulation of hormone secretion (negative and positive feedback regulation)                                    (2 hours)
9. Endocrine disorders                         (10 hours)
10. anterior pituitary gland
11. thyroid gland
12. gonads
13. parathyroid
14. adrenal
15. pancreas
16. posterior pituitary gland

1. Human nutrition (1 credit)

Theory: 1 hour/week

TOPICS

1. Nutrition, calories, calorific value of food, basal metabolism, nitrogen balance         (4 hours)
2. Obesity and weight reduction(2 hours)
3. Vitamins: definition, classification, individual vitamins, chemistry and deficiencies                              (6 hours)
4. Trace elements: definition, occurrence, biological effects, metabolism, deficiencies          (3 hours)

1. Basic Immunology (Two Credits)

Theory: 2 hours/week

TOPICS

1. Properties and overview of immune responses
2. Innate immunity
3. Cells and tissues of the adaptive immune system
4. Antibodies and antigens
5. The major histocompatibility complex
6. Antigen processing and presentation to lymphocytes
7. Antigen receptors and accessory molecules of t lymphocytes
8. Lymphocyte development and the rearrangement and expression of antigen receptor genes
9. Activation of t lymphocytes
10. B Cell activation and antibody production and  switching
11. Immunological tolerance
12. Cytokines
13. Cd Molecules
14. Effector mechanisms of cell-mediated immunity
15. Effector mechanisms of humoral immunity
16. Transplantation immunology
17. Immunity to tumors
18. Diseases caused by immune responses: hypersensitivity and autoimmunity
19. Immediate Hypersensitivity            
20. Congenital and acquired Immunodeficiency

1. Selected topics in clinical chemistry (3 credits)

Theory: 2 hours/week

TOPICS

1. Aspects of molecular biology          (4 hours)
2. Biochemical aspects of hematology (2 hours)
3. Inborn errors of metabolism             (2 hours)
4. Oncogenesis: roles in cancer            (2 hours)
5. Sport chemistry  (2 hours)
6. Bioinformatics   (2 hours)
7. Chemistry of biological fluids                     (2 hours)

Biochemistry PhD Curriculum

1st Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Clinical chemistry I	CHMCch-P1	3	3	4
2	Clinical endocrinology	CHMCen-P1	2	-	2
3	English language	ENGEng-P2	1	-	1
4	Lab. techniques	CHMLtc-P1	2	3	3
5	Toxicology	CHMTox-P1	1	-	1
6	Seminar	CHMSem-P1	1	-	1
Total					12

1. Clinical Chemistry I (4 credits)

Theory: 3 hours/week
Practical: 3 hours/week

TOPICS

1. Abnormal carbohydrate metabolism  (3 hours)
2. Amino acid and protein metabolic disorders(3 hours)
3. Enzymes: Clinical aspects                   (6 hours)
4. Lipoproteins and apoproteins; metabolism and disorders      (6 hours)
5. Water and electrolyte balance disorders(3 hours)
6. Blood gases, acid- base balance and disorders               (3 hours)
7. Liver functions and disorders             (6 hours)
8. Renal functions and disorders            (6 hours)
9. Gastro- intestinal and pancreatic tests         (3 hours)
10. Cardiac ischemia markers                   (3 hours)

1. Physical Biochemistry (2 credits)

Theory: 2 hours/week

TOPICS

 

A. Kinetics of Chemical Reactions:

1. Rate of reaction, the overall order of reaction, Kinetic equations for different orders, half-life of a reaction.
2. Pseudo 1st order reactions, Kinetics of multi-step reactions, Rate constant of reversible reactions.
3. Temperature effects on the velocity of a reaction, Activation energy.
4. Catalysis, Acid-base catalysis, Heterogeneous catalysis.
5. Chemical equilibrium, Equilibrium constant, Factors affecting the chemical equilibrium, LeChatelier's principle.

B. Kinetics of Enzymes:

1. Enzymes, Properties, How enzyme work, factors affecting reaction velocity.
2. Michaelis-Menten equation, Effect of substrate concentration on reaction velocity.
3. Lineweaver-Burk plot, Inhibition of enzyme activity.
4. Problems

C. Kinetics of Adsorption:

1. Adsorption, Types of adsorption, Adsorption isotherms.
2. Langmuir adsorption, types of isotherms.
3. Adsorption of solutes by solids, Adsorption from solution.
4. Wetting agents, Retarded reactions.
5. Heterogeneous reactions, catalytic poisons.
6. problems

1. Toxicology (1 credit)

Theory: 1 hour/week

1. Clinical toxicology
2. Metabolism and toxicity of:
3. Acetaminophen
4. Digoxin
5. Phenobaribitone
6. Phenytoin
7. Amphetamine
8. Methotrexate 
9. Alcohol

4. Lab. techniques (3 credits)
Academic year: 2012-2013
Theory: Two hours/ Week

1. Specimen collection, quality control, method assessment and instrument calibration            (4 hours)
2. Flourimetry, turbidimetry, atomic absorption spectrophotometry                                  (4 hours)
3. Gel electrophoresis                             (2 hours)
4. HPLC                     (2 hours)
5. Affinity and ion exchange chromatography (2 hours)
6. Centrifugation and ultracentrifugation         (2 hours)
7. Tissue homogenization                       (2 hours)
8. Protein determination techniques        (2 hours)
9. PCR and hybridization technique       (2 hours)
10. Electrochemistry   (4 hours)
11. Immunoassay and immunoelectrophoresis                    (4 hours)

Biochemistry PhD Curriculum

2nd Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Clinical chemistry II	CHMCch-P2	2	3	3
2	Clinical nutrition	CHMCnu-P2	2	-	2
3	Metabolic regulation	CHMMre-P2	2	-	2
4	Molecular Biology	CHMMol-P2	2	-	2
5	Selected topics	CHMSto-P2	2	-	2
6	Seminar	CHMSem-P2	1	-	1
7	SPSS	CMRSps-P2	1	-	1
Total					13

1. Clinical Chemistry II

Theory: 2 hours/week
Practical: 3 hours/week

TOPICS

1. Bone metabolism and disorders          (6 hours)
2. Biochemical aspects of hematology            (8 hours)
3. Coagulation chemistry              (6 hours)
4. Lysosomal storage disease        (2 hours)
5. Trace elements in health and disease                    (2 hours)
6. Inborn error of metabolism       (2 hours)
7. Chemistry of body fluids                    (2 hours)
8. Extreme age chemistry              (2 hours)

2. Clinical nutrition (2 credits)
Theory: 2 hours/week
TOPICS

1. Nutritional assessment; biochemical and anthropometric                 (2 hours)
2. Nutrition of pregnant and lactating women           (2 hours)
3. Nutrition of childhood and adolescence       (2 hours)
4. Nutrition of patients with renal failure                  (2 hours)
5. Nutrition of patients with liver disease and hepatic encephalopathy                                                  (2 hours)
6. Nutrition of patients with cancer and other debilitating disease       (2 hours)
7. Nutrition after injury and surgical operations        (2 hours)
8. Parantral nutrition           (2 hours)
9. Obesity, diabetes and weight reduction        (2 hours)
10. Nutrition of cardiovascular disease              (4 hours)
11. Sport nutrition       (2 hours)
12. Nutrition of elderly (2 hours)
13. Nutrition of GIT disease  (4 hours)

 

3. Metabolic regulation (2 credits)
Theory: 2 hours/week
OBJECTIVES: The primary focus of this course is on the pathways of intermediary metabolism by which all cells synthesize and degrade carbohydrates, lipids and nitrogenous compounds. Specifically, we will discuss how metabolic pathways are regulated by effector molecules and by hormones in living systems and to see how organs cooperate in processing food- derived molecules in the fed state, and interact to sustain metabolic activity in the fasted and exercise states. Also, this course will deal with the importance of intracellular compartmentation and gene roles in metabolic regulation.
A. Cellular level – metabolic pathway controls     (4 hours)

1. Metabolic fate of glucose
2. Metabolic fate of fatty acids
3. Metabolic fate of amino acids

B. Common mechanisms of metabolic regulation  (4 hours)

1. Allosteric
2. Covalent modification
3. Enzyme mass
4. Energy charge/ reduction potential of the cell
5. Compartmentation within cell
6. Metabolic specialization of organs
7. Endocrine regulation

C. Major metabolic control sites                            (4 hours)

1. Glycolysis
2. Gluconeogenesis
3. TCA cycle and oxidative phosphorylation
4. Fatty acid oxidation
5. Fatty acid biosynthesis

D. Major metabolic control sites                            (4 hours)

1. Glycolysis
2. Gluconeogenesis
3. TCA cycle and oxidative phosphorylation
4. Fatty acid oxidation
5. Fatty acid biosynthesis

E. Metabolic profiles of organs (5 hours)

1. Brain
2. Skeletal muscle
3. Cardiac muscle
4. Adipose tissue
5. Kidney
6. Liver

F. Metabolic changes during normal, fasting and prolonged starvation     (6 hours)

G. Metabolism during exercise  (4 hours)

1. Type I and type II muscle fibers
2. Metabolism during a short sprint
3. Metabolism during a marathon

H. Genetic level regulation       (3 hours)

4. Molecular Biology (2 credits)

Theory: 2 hours/week

TOPICS

 

A. DNA and RNA

Basic structure components of nucleic acid           

General features of DNA double helix viral nucleic acids & their replication

B. Flow of genetic information

1. Different kinds of cellular RNAs, Hybridization concept

General features of genetic code

Eucaryotic genes are interrupted genes

        
C. Exploring genes (+ Recombinant DNA technology)

1. Definition of recombinant DNA technology
2. Practical application and importance of  
3. Recombinant DNA technology.

Restriction enzymes

Blotting techniques

DNA sequencing methods

DNA probes and uses

DNA vectors

General concepts of PCR and its biomedical application

 
D. DNA replication and repair

DNA grooves and helical forms

DNA topology

DNA polymerase and their action

Mechanism of DNA replication

Mutation and mutagenesis

1. Repair of DNA damage (etiological factors, mechanism of repair)

 
E. Gene rearrangement

1. Recombination, integration.

Transposition and medically important transposons.

Immune diversity and general recombination

 

F. RNA synthesis and splicing

1. RNA polymerases and their biological roles.
2. Mechanism of transcription.

Post-transcriptional modification

1. Enhancer and promotors.
2. Antibiotic action on transcription process.

G. Protein synthesis

Definition, initiators of protein synthesis

Wobble phenomena and t-RNA

Ribosomes and the mechanism of protein synthesis

Differences between procaryotic and eucaryotic translation process.

Antibiotic action on translation

Posttranslational modification of protein

H. Protein targeting

1. Targeting of proteins to intracellular organelles
2. Receptor-mediated endocytosis and its biological significance
3. Targeting proteins for destruction

I. Control of gene expression in procaryotes

1. Operon models for regulation of gene expression
2. Control of translation of ribosomal proteins
3. Control of transcription in viruses

J. Eucaryotic chromosomes and gene expression

1. Structure of chromatin fibers
2. Eucaryotic DNA polymerases
3. Finishing of telomers
4. Control on cell cycle
5. Mitochondrial DNA
6. Repetitive DNA sequences and their importance
7. Hemoglobin gene structure in different
8. Developmental stage

K. Nucleotides &Metabolism of the purine & pyrimidine

L. Cancer, cancer genes, and growth factors

Etiology of cancer

Carcinogenesis

Oncogeneses and protooncogenes

Gene amplification

Polypeptide growth factors are mitogenics

Effect of growth factors on mitogenesis

Effect of growth factors on cell cycle

1. Tumor suppressor genes

5. Selected topics in Clinical Chemistry (2 credits)
Theory: 2 hours/week
TOPICS

1. Biochemistry of Dialysis  (6 hours)
2. Aerobic metabolism       (4 hours)
3. Free radicals, tissue glycation and injury in different diseases (6 hours)
4. Molecular aspects of signaling                     (10 hours)
5. Chemistry of exercise     (4 hours)

Medical chemistry MSc Curriculum

       
1st Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Analytical Chemistry (I)	CMDAch-M1	2	-	2
2	Biochemistry	CMDBio-M1	3	-	3
3	Biostatistics	COMSta-M1	2	-	2
4	English Language	ENGEng-M1	1	-	1
5	Histochemistry	CMDHch-M1	1	-	1
6	Lab. Techniques	CMDLtc-M1	2	3	3
7	Physical Biochemistry (I)	CMDPch-M1	2	-	2
8	Seminar	CMDSem-M1	1	-	1
Total					15

1. Analytical chemistry I (2 credits)

 

Theory: 2 hours/ Week

TOPICS
A. Aqueous solutions

1. Osmosis & osmotic pressure.
2. Determination of molecular weight of proteins.
3. The osmotic behavior of living cells
4. Aqueous solutions of electrolytes
5. Ionization of electrolytes, ionic strength of electrolyte & activity coefficient.
6. Degree of ionization of weak electrolyte.
7. Solubility of salts, solubility products
8. The salt effect, the common ion effect

B. Colloids

1. Nature of colloidal particles & properties of colloids.
2. Classification of colloid systems
3. Preparation of colloidal solutions.
4. The size & molecular weight of colloidal particles
5. The electrical properties of colloids
6. Electrophoresis, isoelectric points of proteins
7. Precipitation of colloids, colloidal electrolytes
8. Emulsions, Gel

2. Biochemistry (3 credits)
Theory: 3 hours/ Week
TOPICS

1. Bioenergetics               (3 hours)
2. Biological membranes and transport mechanisms         (6 hours)
3. Carbohydrates: structure, function, metabolism  (9 hours)
4. Lipids: structure, function, metabolism                (9 hours)
5. Amino acid and protein metabolism                    (9 hours)
6. Creatine and biogenic amine metabolism             (4 hours)
7. Enzymes: nomenclature and classification           (5 hours)

3. Physical Chemistry I (2 credits)
Theory: 2 hours/ Week
TOPICS
A. Kinetics of Chemical Reactions:

1. Rate of reaction, The overall order of reaction,
2. Kinetic equations for different orders, half-life of a reaction.

Pseudo 1st order reactions, Kinetics of multi-step reactions, Rate constant of reversible reactions

Temperature effects on the velocity of a reaction, Activation energy.

Catalysis, Acid-base catalysis, Heterogeneous catalysis.

Chemical equilibrium, Equilibrium constant, Factors affecting the chemical equilibrium, LeChatelier's principle.

B. Kinetics of Enzymes

1. Enzymes, Properties, How enzyme work, factors affecting reaction velocity.
2. Michaelis-Menten equation, Effect of substrate concentration on reaction velocity.
3. Lineweaver-Burk plot, Inhibition of enzyme activity

C. Kinetics of Adsorption

1. Adsorption, Types of adsorption, Adsorption isotherms
2. Langmuir adsorption, types of isotherms
3. Adsorption of solutes by solids, Adsorption from solution
4. Wetting agents, Retarded reactions
5. Heterogeneous reactions, catalytic poisons

4. Laboratory techniques (3 credits)
Theory: 2 hours/week
Practical: 3 hours/week

TOPICS

1. Specimen collection and quality control(4 hours theory)
2. Photometry: Spectro-, Flouro-,flame- and atomic absorption

                      (6 hours theory)

1. Chromatography: Gel filtration and ion-exchange                  (4 hour theory)
2. Electrochemistry                   (4 hours theory)
3. Electrophoresis technique     (2 hours theory)
4. Radio-immunoassay and ELISA technique                   (4 hours theory)
5. Centrifugation and ultra- centrifugation(3 hours theory)
6. Tissue homogenization         (2 hours theory)

 

2nd Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Analytical Chemistry (II)	CMDAch-M2	2	-	2
2	Clinical Chemistry	CMDCch-M2	2	3	3
3	Physical Biochemistry (II)	CMDPch-M2	2	-	2
4	Research Methodology	COMRme-M2	1	-	1
5	Seminar	CMDSem-M2	1	-	1
Total					9

1. Analytical Chemistry II (2 credits)

Theory: 2 hours/week
TOPICS

1. The nature of chelation, Equilibria in solution of chelating ligands. Conditions for chelation.
2. Chelation in biological system, Macrocyclic antibiotics, Metal complexes of nucleotides, Metalloproteins.
3. Heam iron proteins, Vitamin B12 & related compounds, Lead poisoning, Arsenical poisoning, chelating agents in the treatment of cancer.
4. Theory of complexation titrations, stability of complexes, A simple complexation titration some practical considerations.
5. Complexones, EDTA complexes, Titration curves, Types of EDTA titrations, Masking & demasking agents, Evaluation of complexation titrimetry.
6. Titrations based on redox reactions, Redox titration curves, Sketching a redox titration curve, Evaluating the end point.
7. Separation techniques: based on size, based on mass or density, based on complexation reactions (masking), based on a change of state, based on a partitioning between phases.
8. Liquid-liquid extractions.
9. Spectroscopic techniques: nature of light, electromagnetic spectrum, UV-visible absorption spectroscopy.
10. Equipment used in absorption spectroscopy. Applications of absorption spectroscopy.
11. Flame spectrometry, theory, instrumentation, combustion flames, nebuliser-burner system. Non-flame techniques, resonance line sources, monochromator, detectors, interferences, single beam atomic absorption spectrophotometers, double beam atomic absorption spectrophotometers.
12. Evaluation methods: Preparation of sample solution, preparation of standard solutions, safety practices.
13. Selected determinations: Mg & Ca in tap water determination of trace lead in ferrous alloy.

1. Physical Biochemistry II (2 credits)

Theory: 2 hours/ Week
TOPICS
A. Thermodynamics

1. Background, Entropy, Free energy, spontaneous reactions, standard states & standard functions.
2. Enthalpy change, Free energy change, relationship between standard free energy & equilibrium constant.
3. Standard free energy for reaction involves H+ ions, temperature dependence of standard free energy, Free energy change under non- standard conditions.
4. Thermodynamics of reactions in aqueous solutions.
5. The application of thermodynamics to Biochemistry.
6. High energy compounds, mechanisms of some reactions.

B. Redox

1. Effect of pH on redox potential.
2. Potentiometric titration, Redox indicators.
3. Sluggish redox couples.
4. Electron transport & the respiratory chain.

1. Clinical Chemistry (3 credits)

Theory: 2 hours/ Week
Practical: 3 hours/ week

TOPICS

1. Carbohydrate disorders      (4 hours)
2. Lipid disorders  (2 hours)
3. Amino acids and protein disorders             (4 hours)
4. Liver function tests             (2 hours)
5. Renal function tests            (2 hours)
6. Water and electrolyte regulation                 (6 hours)
7. Clinical enzymology           (4 hours)
8. Extreme age chemistry       (4 hours)

Medical chemistry PhD Curriculum

       
1st Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Clinical chemistry I	CMDCch-P1	3	3	4
2	English language	ENGEng-P1	1	-	1
3	Lab. techniques	CMDLtc-P1	2	3	3
2	Physical Biochemistry	CMDPch-P1	2	-	2
3	Seminar	CMDSem-P1	1	-	1
4	Toxicology	CMDTox-P1	1	-	1
Total					12

           

• Clinical Chemistry I (4 credits)

Theory: 3 hours/week
Practical: 3 hours/week

TOPICS

• Abnormal carbohydrate metabolism     (3 hours)
• Amino acid and protein metabolic disorders   (3 hours)
• Enzymes: Clinical aspects     (6 hours)
• Lipoproteins and apoproteins; metabolism and disorders      (6 hours)
• Water and electrolyte balance disorders(3 hours)
• Blood gases, acid- base balance and disorders               (3 hours)
• Liver functions and disorders                (6 hours)
• Renal functions and disorders               (6 hours)
• Gastro- intestinal and pancreatic tests            (3 hours)
• Cardiac ischemia markers     (3 hours)

• Physical Biochemistry (2 credits)

Theory: 2 hours/week

TOPICS

A. Kinetics of Chemical Reactions:

• Rate of reaction, the overall order of reaction, Kinetic equations for different orders, half-life of a reaction.
• Pseudo 1st order reactions, Kinetics of multi-step reactions, Rate constant of reversible reactions.
• Temperature effects on the velocity of a reaction, Activation energy.
• Catalysis, Acid-base catalysis, Heterogeneous catalysis.
• Chemical equilibrium, Equilibrium constant, Factors affecting the chemical equilibrium, LeChatelier's principle.

B. Kinetics of Enzymes:

• Enzymes, Properties, How enzyme work, factors affecting reaction velocity.
• Michaelis-Menten equation, Effect of substrate concentration on reaction velocity.
• Lineweaver-Burk plot, Inhibition of enzyme activity.
• Problems

C. Kinetics of Adsorption:

• Adsorption, Types of adsorption, Adsorption isotherms.
• Langmuir adsorption, types of isotherms.
• Adsorption of solutes by solids, Adsorption from solution.
• Wetting agents, Retarded reactions.
• Heterogeneous reactions, catalytic poisons.
• problems

• Toxicology (1 credit)

Theory: 1 hour/week

• Clinical toxicology
• Metabolism and toxicity of:
• Acetaminophen
• Digoxin
• Phenobaribitone
• Phenytoin
• Amphetamine
• Methotrexate 
• Alcohol

4. Lab. techniques (3 credits)
Academic year: 2012-2013
Theory: Two hours/ Week

• Specimen collection, quality control, method assessment and instrument calibration                                    (4 hours)
• Flourimetry, turbidimetry, atomic absorption spectrophotometry                                                          (4 hours)
• Gel electrophoresis               (2 hours)
• HPLC                                             (2 hours)
• Affinity and ion exchange chromatography    (2 hours)
• Centrifugation and ultracentrifugation            (2 hours)
• Tissue homogenization         (2 hours)
• Protein determination techniques           (2 hours)
• PCR and hybridization technique          (2 hours)
• Electrochemistry                   (4 hours)
• Immunoassay and immunoelectrophoresis                    (4 hours)

Medical chemistry PhD Curriculum

2nd Semester

Subjects		Code	Hours/week		Credits
			Theory	Practical
1	Applied spectroscopy	CMDAss-P2	3	-	3
2	Clinical Chemistry II	CMDCch-P2	2	3	3
3	Pharmaceutical Chemistry	CMDPcc-P2	1	-	1
4	Seminar	CMDSem-P2	1	-	1
5	SPSS	CMRSps-P2	1	-	1
6	Surface chemistry	CMDSch-P2	3	-	3
Total					12

• Clinical Chemistry II (3 credits)

Theory: 2 hours/week
Practical: 3 hours/week

TOPICS

• Bone metabolism and disorders     (6 hours)
• Biochemical aspects of hematology       (8 hours)
• Coagulation chemistry          (6 hours)
• Lysosomal storage disease    (2 hours)
• Trace elements in health and disease     (2 hours)
• Inborn error of metabolism   (2 hours)
• Chemistry of body fluids                (2 hours)
• Extreme age chemistry          (2 hours)

 

• Medical radiochemistry (2 credits)

Theory: 2 hours/week

• Natural radioactivity, nuclear medicine.
• Types of radiation.
• Biological effects of radiation.
• Detecting ionizing radiation.
• Half-Life, radiation dosages.
• Medical uses of radioactive isotopes.
• Cancer therapy using radiation.
• Nuclear waste disposal.
• Nuclear medicine, making isotopes for medical applications.
• Rate of radioactive decay.
• Effect of ionizing radiation on living matter.

• Pharmaceutical Chemistry (2 credits)

Theory: Two hours/Week
  TOPICS

• Drug distribution
• Parenteral  administration
• Protein binding
• Tissue depots
• Drug metabolism
• Excretion
• Receptors
• Acid- base properties
• Pharmacokinetics
• Bioavailability
• Design of the study
• Sampling
• The choice of the method of analysis
• Data analysis

• Surface chemistry (2 credits)

Theory: 2 hours/week
TOPICS

• Adsorption, influence of temperature & pressure.
• Adsorption isotherms.
• Types of adsorption.
• Adsorption at surfaces of solutions, Gibbs equation.
• Adsorption by solids from solution, adsorption & concentration, surface area of adsorbents.
• Chromatographic adsorption.
• Zeta potential electroosmosis.
• Determination of surface area of adsorbents, applications of adsorption.
• Retarded reactions, the order of heterogeneous reactions.
• Effect of temperature on heterogeneous reactions.
• Catalytic poisons.