Anti—Pathogenic Candida Spp. Activity Determination Via Lactobacillus Spp. Isolation And Identifications Using Conventional And Molecular Methods
ازهار عبد الفتاح ابراهيم
Authors : Hawraa F. H. AL-abedi*, Azhar A.F.AL-Attraqchi**, Bassam Y. Khudaier***
Two Hundred and fifty samples of cow's milk from different parts of the province of Basrah were collected from clinical and subclinical mastitis reported using the California mastitis test between March 2018 and September 2019 and examined using conventional PCR assay, Candida species was identified in 116/250 (46.4%). Based on conventional method and ID - Yst card system Vitek 2, Candida albicans was the predominant 60/116 (51.7%), followed by Candida parapsilosis 15/116 (12.9%). Concerning the results of PCR amplification of 18S rRNA gene for identification of C. albicans and C. parapsilosis, this gene was present in 60 samples in C. albicans, and in 15 of C. parapsilosis. Lactobacillus are an industrially important group of probiotic organisms that play an important function in human health through inhibiting dangerous and pathogenic bacteria growth, boosting immune function, and increasing resistance to infection. Ten out of 250(4%) Lactobacillus isolates were obtained from apparently healthy cow milk samples. Lactobacillus isolates were identified according to phenotypic characterization and molecular technique using PCR (16S rRNA) and sequencing, it was seen that L. acidophilus formed 5 isolates (50%), L.amylovorus was three (30%), while Basrah Journal of Veterinary Research,Vol.19, No.3, 2020. Proceeding of the 17th International Conference. College of Veterinary Medicine. University of Basrah. Iraq 131 L.crisaptus formed only two (20%) only. The results of this study revealed that the BLAST analysis at the NCBI gene bank gave 99.39% homology with L. acidophilus, 99.19% homology with L.crispatus and 97.59% with L. amylovorus. In vitro antimycotic activity of probiotic bacteria (Lactobacillus) against C. albicans and C. parapsilosis using agar well diffusion methods was adapted. The cell-free neutralized supernatant (CFS) of Lactobacilli (105,106,107) were inhibited the growth of pathogenic C.albicans and C. parapsilosis. It was also noticed that, L. acidophilus showed the strongest antifungal activities against pathogenic C. albicans and C.parapsilosis with different degrees of inhibition zones in comparsion with each of L.crispatus and L. amylovorus, meanwhile, L. amylovorus revealed strongest antifungal activity against pathogenic C.parapsilosis.

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