Abstract:
Background: Methicillin resistance Staphylococcus aureus (MRSA) infection related to
community has been enhanced through the world. One of the significant cytotoxins
elaborated by a few strains of S. aureus is the Panton Valentine leukocidin (PVL),
coded by two genes, lukS- PV and lukF-PV which is element of toxin that created
pores in the membranes of cells, the role of PVL in the disease process severity of
Staphylococcus aureus till known is debated.
Amis: This study was planned to detect the Panton Valentine leucocidin (PVL)genes
and MRSA isolated from Burn wound infection in community and hospital acquired
settings.
Material and methods: Cross-sectional study involved 200 patients who presented with
burn wounds from third-degree or fourth-degree. The bacteria from all samples were undergone to Cefoxitin disc diffusion testing utilization a 30 μg cefoxitin disc for methicillin resistant determination. Following DNA extraction, conventional polymerase chain reaction (PCR) was used to detect PVL genes.
Results: Twenty-eight samples were positive culture for Staph. aureus, of which 19 (67.8%) patients presented with CAI, and 9 (32.1%) belong Hospital acquired infection A total of 23 (82.1%) showed methicillin resistance. Out of 28 isolates, 7(25%) isolates were harboring PVL gene, all of which were Methicillin resistance community acquired
infection. None of S. aureus isolates from hospital acquired infection had PVL.
Conclusion: This study indicated high prevalence of PVL among community acquired infection MRSA isolates, and the lack of this gene in Hospital acquired infection.
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2020
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