Abstract
Objective: To determine the possible genetic polymorphism of rs6265 genetic variants of BDNF gene, and its relationship with Major Depressive Disorder pathophysiology. Method: Case control study, that the blood samples had taken from 120 participants were collected from 60 patients diagnosed as MDD according to DSM-V criteria by psychiatrist and 60 normal controls not undergo any mood disorders and all participants had did not undergo any chronic disease. The collected blood divided into two, one for ELISA to estimate BDNF concentration and the other one to PCR for genotyping study. Results: Using statistical analysis for the evaluation the serum levels of BDNF and genetic variation, the levels of BDNF concentrations in depressed patients were estimated and there were significantly lower than healthy control group which was 729.12 pg.ml-1 in patients with depression compared with healthy control group which was 1169.5 pg.ml-1 p value (p<0.005). CC wild allele in patients was (53%), TT homozygous mutant was (22%) and the heterozygous mutant CT was (25%) of patient while in controls was CC (50%), TT (23%) and CT (27%). Odd ratio for CC, CT and TT genotypes in the studied groups, were 1.0, 0.8789 and 0.8705 respectively, which mentioned that C>T polymorphism was protective but not a risk factor for incidence of Major Depressive Disorder. Conclusions: Brain Derived Neurotrophic Factor protein that estimated by ELISA method was decreasing significantly in depressive patients when compared with healthy control group. In other hand, there was non-significant relationship of genotypes rs6265 with Major Depressive Disorder pathophysiology in Iraqi people.
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5/11/2022
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