Pseudomonas aeruginosa is the most frequent etiological agent of otitis externa. In this study
polymerase chain reaction was used to investigate the genotypic properties and genetic relationship between P.
aeruginosa obtained from human and dogs with otitis externa in Iraq. Seventeen (17%)P. aeruginosawere
isolated from 100 ear swabs of human and six isolate (6%) from 100 ear swabs of dogs by conventional
methods (culture and biochemical tests). A total of 23 P. aeruginosa isolate from human and dog were subjected
to PCR to detect the Exo A gene by amplifying a 396-bp region using the same primer. Results showed that,
six(26 %) and two (8.6 %) isolates from human and dogs give positive product for exo A gene respectively. in
conclusion, there is a genetic relationship between P. aeruginosa isolated from human and dogs in Iraq.
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(Sep -Oct. 2014)
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