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The Frequency of Enterococcus species and Resistance Genes (ant (3 “)-III, and van A) in patients on Hemodialysis with Urinary Tract Infections
ثناء رشيد عبد الرحمن
Authors : Ola Sabah Mohammed, Thanaa Rasheed Abdulrahman, Arif Sami Malik, Shatha Hussain Ali
Urinary tract infections (UTIs) are common in dialysis patients, associates with increasing rate of complications, and may be difficult to diagnose due to the subclinical presentation. Enterococcus spp. is a frequent cause of complicated UTI in patients with risk factors. The current study aimed to estimate the percentage of Enterococcus species associated with urinary tract infection in hemodialysis patients and detection the resistance genes (ant (3“)-III, ant (6)-I and van A) in isolated MDR and XDR Enterococcus species. A total of (120) urine samples, 60 from hemodialysis patients with symptomatic urinary tract infections (UTIs) and 60 from UTI patients without hemodialysis was included in this study; The isolation of bacteria was performed by culturing on MacConkey agar, Blood agar, HiCrome™ UTI Agar and Brain heart infusion broth and the identification by Vitek-2 system. The detection of resistance genes (ant (3“)-III, and van A) was done by conventional polymerase chain reaction (PCR). The percentage of Enterococcus faecalis in UTI patients was 16.66% (11.66% in patients with hemodialysis and 5.0% in patients without hemodialysis). Twelve bacterial isolates (85.71%) out of 14 from patients with HD possessed ant (3“)-III gene compared with 4 isolates out of 6 (66.67%) from patients without HD

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2023