On Thursday, June 6, 2024, at 9:00 AM, the PhD candidate Ismail Nassif Jassim successfully defended his dissertation in the Discussion Hall of the Department of Chemistry and Biochemistry/Medical Biochemistry. His dissertation, titled: **Synthesis of Gold Nanoparticles by Green-Cold Plasma and Its Effect on Breast Cancer Cells** aimed to: 1. Prepare gold nanoparticles from their salts and combine them with Adriamycin using a green-cold plasma jet system. 2. Study the effects of gold nanoparticles, Adriamycin, and AuNPs-AD on breast cancer cell lines in vitro using cytotoxicity assays and inhibitory genetic biomarkers. 3. Investigate the synergistic effect of the combination of gold nanoparticles and Adriamycin in vitro. ### Results: 1. Characterization techniques confirmed distinctive features for each, with visible and UV light results revealing peaks at specific wavelengths. FESEM results showed that the produced gold particles were amorphous spheres ranging in size from 38-76 nm. 2. The in vitro study included breast cancer cell lines (MCF7, AMJ13) and a normal cell line (REF). The microplate wells were subjected to various treatments, and growth inhibition and gene expression were assessed. The highest growth inhibition for MCF7 and AMJ13 cells was observed with AuNPs-AD. 3. Gene expression studies indicated different mechanistic responses of apoptosis in the treated cells in vitro when exposed to AuNPs-AD. MCF7 cells showed an upregulation in P53 during 48 hours of exposure and a decrease in P21 throughout the entire exposure period. ### The discussion committee consisted of: - Prof. Dr. Omar Farouk Abdul Rashid - Chairman - Prof. Dr. Mohammed Jassim Hamza - Member - Prof. Dr. Ahmed Hashim Hussein - Member - Assoc. Prof. Dr. Qahtan Adnan Mahdi - Member - Assoc. Prof. Dr. Duha Salem Naama - Member - Prof. Dr. Istabraq Abdul Rasool Kuweiri - Member and Supervisor - Assoc. Prof. Dr. Ban Hassan Adel - Member and Supervisor The dissertation was accepted with distinction. 
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