**On Sunday, June 9, 2024, the PhD defense of the doctoral student (Siham Abdul Sahib Mohsen) was held at 9:00 AM in the discussion hall of the Department of Chemistry and Biochemistry/Clinical Biochemistry for her dissertation titled:** **"Study The Relationship Between Oxidative Stress, MC1R Gene Polymorphism, and Tyrosinase Level in Female with Melasma in Baghdad City"** **Purpose of the study:** To estimate the levels of tyrosinase, 8-OHdG, and glutathione in the serum in relation to melasma severity as biomarkers for diagnosis and treatment. To determine the relationship between MC1R gene polymorphism, tyrosinase, 8-OHdG, and glutathione in melasma. Additionally, to investigate the correlation between increased tyrosinase, elevated 8-OHdG levels, decreased glutathione, and SNPs rs1805006 and rs772551710 in MC1R gene polymorphism. Moreover, the study aims to explore a new rapid diagnostic method for melasma based on the biomarkers and SNPs measured using Tetra-primer ARMS-PCR protocol. **Results:** This research found a significant increase in tyrosinase concentration along with decreased antioxidant levels in women with melasma. The mean and standard deviation in serum for tyrosinase, 8-OHdG, and glutathione among melasma patients and the control group were evaluated using an independent t-test. The mean and standard deviation according to the severity of melasma were ±SD (7.36±0.19) U/ml, (3.80±0.22) ng/ml, and (18.15±0.81) ng/ml, respectively, with a p-value <0.0001. Furthermore, the rs1805006 gene showed a significant (CA) mutation at a value of (p=0.0004*) with an OR = 4.67 (83.33% Cl: 2.0052 to 10.9106). Similarly, the second gene, rs772551710, showed a significant (II) mutation at a value of (p = 0.002*) with an OR = 3.28 (76% Cl: 1.5008 to 7.1955). The findings of this study strongly indicate that these biomarkers play a role in the formation and progression of melasma. **The discussion committee consisted of:** - Prof. Dr. Mohammed Omran Hamzah - Chair - Prof. Dr. Moataz Sabah Ahmed - Member - Assist. Prof. Dr. Hassan Nasser Mohsen - Member - Assist. Prof. Dr. Majid Arshid Sabah - Member - Assist. Prof. Dr. Raed Jasim Mohammed - Member - Prof. Dr. Estabraq Abdul Rasool Kuwairi - Member and Supervisor - Assist. Prof. Dr. Iqbal Ghalib Farhood - Member and Supervisor The thesis was successfully accepted with distinction. 
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