|
Postgraduate
|
MSc Microbiology
1st Semester
Subjects |
Code |
Hours/week |
Credits |
Theory |
Practical |
1 |
Bacteriology |
MICBac-M1 |
1 |
- |
1 |
2 |
Biostatistics |
COMSta-M1 |
2 |
- |
2 |
3 |
English language |
ENGEng-M1 |
1 |
- |
1 |
4 |
Immunology |
MICImm-M1 |
2 |
3 |
3 |
5 |
Molecular biology |
MICMol-M1 |
2 |
- |
2 |
6 |
Virology |
MICVir-M1 |
2 |
3 |
3 |
7 |
Pathology |
PATMic-M1 |
1 |
- |
1 |
8 |
Biochemistry |
CHMMic-M1 |
1 |
- |
1 |
Total |
14 |
2nd Semester
Subjects |
Code |
Hours/week |
Credits |
Theory |
Practical |
1 |
Bacteriology |
MICBac-M2 |
2 |
3 |
3 |
2 |
Mycology |
MICMyc-M2 |
1 |
3 |
2 |
3 |
Parasitology |
MICPar-M2 |
2 |
3 |
3 |
4 |
Research methodology |
COMRme-M2 |
1 |
- |
1 |
5 |
Seminar |
MICSem-M2 |
- |
- |
- |
Total |
10 |
PhD Microbiology
1st Semester
Subjects |
Code |
Hours/week |
Credits |
Theory |
Practical |
1 |
Bacteriology |
MICBac-P1 |
1 |
- |
1 |
2 |
English language |
ENGEng-P1 |
1 |
- |
1 |
3 |
Molecular biology |
MICMol-P1 |
2 |
- |
2 |
4 |
Parasitology |
MICPar-P1 |
2 |
3 |
3 |
5 |
Seminar |
MICSem-P1 |
- |
- |
- |
6 |
Immunology |
MICImm-P1 |
2 |
3 |
3 |
Total |
10 |
2nd Semester
Subjects |
Code |
Hours/week |
Credits |
Theory |
Practical |
1 |
Bacteriology |
MICBac-P2 |
3 |
3 |
4 |
2 |
Gene therapy |
MICGth-P2 |
2 |
- |
2 |
3 |
Mycology |
MICMyc-P2 |
2 |
3 |
3 |
4 |
Seminar |
MICSem-P2 |
1 |
- |
1 |
5 |
SPSS |
CMRSps-P2 |
1 |
- |
1 |
6 |
Virology |
MICVir-P2 |
2 |
3 |
3 |
Total |
14 |
Aims of the Study:
The MSc in Medical Microbiology is a one-year full time course designed to provide MSc students with knowledge in two courses, stressing the challenges posed by pathogens and how they shape immune responses.
Objectives of the Study:
Students will learn about clinically relevant pathogens through an introductory module on Medical Microbiology and specialized courses on Bacteriology, Immunology, Virology, Parasitology, Mycology and Molecular biology.
Provision for the development of practical research skills will be offered by each course, preparing the students for Research Projects leading to a dissertation.
Medical Bacteriology:
M.Sc First Course: 1 credit = 1 theory lecture / week
|
|
Introduction Classification and Taxonomy |
1. |
Bacteria compared with other microorganisms |
2. |
Structure of bacterial cell |
3. |
Sterilization and disinfection |
4. |
Bacterial nutrition, culture media |
5. |
Normal,microbial flora of the human body |
6. |
Growth of Bacteria |
7. |
Microbial metabolism,energy and biosynthesis |
8. |
Microbial Genetics |
9. |
Antimicrobial drugs/ mechanism of action |
10. |
Antimicrobial drugs/resistance |
11. |
Bacterial vaccines |
12. |
Virulence and pathogenesis |
13. |
Bacterial Toxins |
14. |
Bacteria Enzymes |
15. |
M.Sc Second Course:
Credit: 3 units = (2 hrs theory+3 hrs practical)/week
|
|
1. |
Gram- positive cocci |
2. |
Gram- negative cocci |
3. |
Gram- positive rods |
4. |
Sporulated bacteria/ Gram- positive rods |
5. |
Gram- negative rods (enteric bacteria) |
6. |
Pseudo ,Bordetella and Haemophillus. |
7. |
Brucella spp. |
8. |
Comma shaped and spiral bacteria |
9. |
Mycobacteria |
10. |
Mycoplasma |
11. |
Chlamydiae |
12. |
Rickettsiae |
13. |
Actinomycetes, Nocardia |
14. |
Treponema |
15. |
Zonootic Bacteriology |
Practical Sessions:
|
|
1. |
Specimen collection and management |
2. |
Direct & general examination of specimens. |
3. |
Antimicrobial susceptibility |
4. |
Gram- positive cocci |
5. |
Gram- negative cocci |
6. |
Gram- positive rods |
7. |
Gram- negative rods (enteric bacteria) |
8. |
Comma shaped and spiral bacteria |
9. |
Brucella, pseudomonas |
10. |
Mycobacteria |
11. |
Actinomycetes, Nocardia |
12. |
Mycoplasma |
13. |
Chlamydia, Rickettsia Treponema, |
Medical Immunology
One course: first
Credit: 3 units = (2 hrs theory+3 hrs practical)/week
1- |
Introduction to immune system
-Antigen Ag, Foreign (Pathogen) Antigens
-CD Antigen Markers |
2 |
-B Cell Development
-Generation of B Cells
-Regulation of B Cell Development
-Positive Selection of B Cells
-Negative Selection of B Cells
-B Cell Heterogeneity
Acquired immune response:-Abs |
3 |
-T Cell Development
-Generation of T Cells
-Positive Selection of T cells
-Negative Selection of T Cells
T Cell Activation
Properties of Effector Cells
Cytotoxic T Cells
Macrophage Activation by Th1 (Inflammatory T) Cells |
4- |
Complement : Functions, Cascades and Regulationof Function |
5- |
Cytokines |
6- |
MHCImmune system cells cooperation |
7- |
Infection and Immunity |
8- |
Tumor Immunology |
9- |
Hypersensitivity typeI, II |
10- |
Hypersensitivity type III,IV |
11- |
Autoimmunity Part 1 |
12- |
Autoimmunity Part 2 |
13- |
Immune Deficiency diseases |
14- |
Transplantation |
15- |
Vaccines |
Practical Sessions:
- Introduction
|
- Ab-Ag reaction (precipitation)
|
- Electrophoretic Techniques
|
- Ab-Ag reaction (agglutination)
|
- Ab-Ag reaction (hemagglutination)
|
- Ab-Ag reaction (complement fixation)
|
- Complement system assessment (CH50% and CH100%)
|
- Ab-Ag reaction (ELISA)
|
- Ab-Ag reaction (immunoflourescence testandRIA)
|
- Cell isolation, Cell counting and functional assessment
|
- Immunocytochemistry
|
- Immunohistochemistry
|
- Cross-matching and HLA-typing
|
- In situ Hybridization
|
Medical Virology:
One course: first
Credit: 3 units = (2 hrs theory+3 hrs practical)/ week
- General prosperities and classification of viruses
|
- Viral replication and viral genetics
|
- Cultivation of viruses, effect of virus on host cells, pathogenesis of viral disease (acute, chronic, latent, and slow viral infection).
|
- Prevention and treatment of viral infections: interferon, antiviral chemotherapy and viral vaccines.
|
- DNA Viruses, enveloped and non-enveloped.
|
- RNA Enveloped Viruses: Orthomyxoviruses
|
- Paramyxoviruses
|
- Hepatitis Viruses
|
- Rhabdovirus: RNA non-enveloped viruses
|
- Reo, Corona virus, SARS
|
- Retroviruses
|
- Oncogenic viruses
|
Practical Sessions:
- Introduction to Practical Virology
|
- Methods of diagnosis of viral infections
|
- Preparation of tissue culture, clinical specimens’ collection and handling.
|
- Inoculation of clinical sample in tissue culture, in embryo-egg, and in lab. Animals.
|
- Immuno-Electon Microscopy (IEM).
|
- Immunocytochemistry (ICC).
- Immunohistochemistry (IHC).
|
- In situ hybridization (ISH).
|
- Polymerase chain reaction (PCR)
|
- Polymerase chain reaction (PCR)
|
- Indirect Methods: Serology.
|
Medical Parasitology:
One course: Second
Credit: 3 units = (2 hrs theory+3 hrs practical)/ week
- Host-parasite origin, adaptation to parasitism, definitions and classification. Fate of parasite and evasion of the immune system, effect of parasite on the host.
|
- Pathology and pathogenesis of intestinal nematodes.
|
- Anemia in helminth infections with special reference to hookworm anaemia.
|
- Hydatid disease.
|
- Vaccines in Helminthology. V.L.M. Eosinophilia.
|
- Bladder carcinoma and bilharzia.
|
- Intraspecific variations and epidemiolog
|
- Medical Protozology :Introduction, definitions, classification, origin of parasitism
|
- Entamoebahistolytica, pathogenic free-living amoebae
|
- Giardiasis: pathogenesis of chronic diarrhea and impact on child health and development.
|
- Intraspecific variations, method used and epidemiology.
|
- Vaccination with special reference to malaria and leishmaniasis.
|
- Drug resistance in malaria. AIDS and parasitic infections, a new concept of parasitic zoonoses
|
- Immunity to protozoal infections.
- Congenital Toxoplasmosis.
|
- Medical Insects
|
Practical Sessions
- Introduction,fecal examination
|
- Enterobius,Trichuris, Strongyloides, Ascaris
|
- Hookworms
|
- Echinococcus spp.
|
- Trichinella , Filaria, larva migrans,
|
- Schistosoma spp.
|
- Cultivation of helminthes Preservation of helminthes.
|
- Blood film and stool examination
|
- Introduction, faecal examination, Entamoeba. Other amoebae
|
- Flagellates, ciliates
|
- Culture methods used in Protozoology.
|
- Leishmania, Trypanosoma.Malaria (Plasmodium)
|
- Preservation of protozoa
|
- Animal inoculation as a method of diagnosis. Staining method.
|
- Medical Insects
|
Medical Mycology
Course: one course second
Credits: two units= (1 hr theory + 2 hrs practical sessios) / week
- The fungi: An introduction of different pathogenic fungi, their general properties, immunity to fungi & predisposing factors.
|
- Mycetoma; Etiology, Epidemiology & ecology, Clinical manifestations, Pathology & immunology,Treatment, & lab. DX.
|
- Dermatophytosis: Etiology, Epidemiology &ecology, Clinical manifestations, Pathology &Iimmunology,Treatment, & lab. DX.
|
- Candidiasis; Etiology, Epidemiology &Ecology, Clinical manifestations, Pathology &Immunology,Treatment, & lab. DX.
|
- Cryptococcosis; Etiology, Epidemiology & ecology, Clinical manifestations, Pathology & immunology,Treatment, & lab. DX.
|
- Histoplasmosis; Etiology, Epidemiology &ecology, Clinical manifestations, Pathology & immunology,Treatment, & lab. DX.
|
- Sporotrichosis; Etiology, Epidemiology &ecology, Clinical manifestations, Pathology & immunology,Treatment, & lab. DX.
|
- Blastomycosis; Etiology, Epidemiology &ecology, Clinical manifestations, Pathology & immunology,Treatment, & lab. DX.
|
- Miscellaneous Fungi
|
- Mycotoxicosis
|
- Antimycotic Therapy
|
- Topics presented by students
|
Practical Sessions
- An introductory review of the important pathogenic fungi, &How to diagnose these fungi
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
- Data show of the pathogenic agent & The types of lesions it produced.
|
Molecular Biology:
Credits: 2 units: 2 theory lectures /week
- Introduction and DNA replication
|
- Gene expression: mechanisms of gene expression and gene expression control (operons)
|
- Microbial genetics:
- -Transformation
- -Conjugation
- -Transduction
|
- Molecular biology applications and tests:
- gel electrophoresis
- - RFLP
|
- Hybridization techniques
- - Gene cloning
|
- PCR: Optimization of PCR
|
- Genetic Mutations
|
- Genetic variations
|
- Cell Cycle
|
- Genetic Check points
|
- Introduction to Cytogenetic
|
- Mitochondrial DNA
|
Postgraduate PhD Curriculum Medical Microbiology Department
Medical Bacteriology:
First Course:
Credit = 1 Credit: 1 theory lecture /week
- Taxonomy,classification, Nomenclature &Identification
|
- Bacterial cell
|
- Environmental and Nutritional requirements
|
- Growth and division
|
- Metabolism
|
- Normal flora of human body
|
- Bacterial genetics, cloning and gene expression
|
- Extra chromosomal elements
|
- Mutations and gene transfer
|
- Outbreak investigations and diseases management
|
- Hospital infection
|
- Virulence and pathogenesis
|
- Bacterial toxins
|
- Bacterial antagonism:Bacteriocins
|
- Epidemiology of infectious diseases
|
Second Course:
Credits: 3 credit = (2hr theory lectures+ 2 hr practical)/week
- Upper respiratory tract infection
|
- Lower respiratory tract infection
|
- Gastro-intestinal system infants
|
- Gastro-intestinal system adults
|
- Genito-urinary system females
|
- Genito-urinary system males.
|
- Microorganisms of skin diseases
|
- Mucous membrane diseases
|
- Infections of muscle, joints, and bone
|
Infections of eye |
- Infections of blood
|
- Zoonotic bacterial diseases
|
- Blood and CSF/ Swabs & soft tissue specimens
|
Practical Sessions
- Specimens/Collection & handling of specimens.
|
- Direct & general examination of specimens/ Staining.
|
- Media & Pure culture
|
- Sterilization
|
- Antimicrobial Susceptibility.
|
- General examination & testing for M.O. encountered in the urine.
|
- General examination & testing for M.O. encountered in the stool
|
- General examination & testing for M.O. encountered in the blood
|
- General examination & testing for M.O. encountered in the CSF
|
- General examination & testing for M.O. encountered in the sputum
|
- Swabs (wound, soft tissues, urethra & vaginal).
|
- Genetic probes in diagnostic microbiology
|
Medical Immunology
First Course:
Credits: 3 credit = (2 hr theory lectures+ 2 hr practical)/week
- T-lymphocyte-types activation
|
- T-regulatory and B-regulatory, cross presentation
|
- Dendritic cells and their role in immune response
|
- Apoptosis
|
- CPG and their role in immunity
|
- Signal transduction in T & B cells
|
- Heat shock proteins and immune response
|
- Role of different stress and IR
|
- Endocrine and IS
|
- HLA and diseases
|
- Complement
|
- Immuno-regulation
|
- Vaccination
|
Practical Sessions:
- Ab-Ag reaction (precipitation)
|
- Electrophoretic Techniques
|
- Ab-Ag reaction (agglutination)
|
- Ab-Ag reaction (hemagglutination)
|
- Ab-Ag reaction (complement fixation)
|
- Complement system assessment
- (CH50% and CH100%)
|
- Ab-Ag reaction (ELISA)
|
- Ab-Ag reaction (immunoflourescence testandRIA)
|
- Cell isolation, Cell counting and functional assessment
|
- Immunocytochemistry
|
- Immunohistochemistry
|
- Cross-matching and HLA-typing
|
- In situ Hybridization
|
Second Course:
Credit: 1 credit = 1 theory lecture /week
- Tumor immunology, basic theories for arising of tumors
|
- Molecular aspects in cell transformations
|
- Immune surveilence and tumor escape mechanisms
|
- Tumor markers-adjuvants and immunotherapy for cancers
|
- Autoimmunity-part- I
|
- Autoimmunity-part-II
|
- Mid-term exam
|
- Transplantation-Part- I
|
- Transplantation-Part- II
|
- Hypersensitivity-part –I
|
- Hypersensitivity-part –II
|
- Reproductive immunology
|
- Immunity to infection part –I
|
- Immunity to infection part –II
|
- IDD
|
Medical Virology
Second Course:
- Introduction, definitions in virology and viral replication cycle
|
- Chemical composition of viruses, viral genetics, interactions among viruses
|
- Interferons (as antivirals),
-viral vaccines,
-antiviral-drugs (mechanisms of action) |
- DNA enveloped viruses (Herpes viruses)
-Latent viral infections, HSV-1 latent in vestibular ganglia,
-Human herpes virus 6 in non-neurological diseases,
-oncogenesis of human herpes virus 8 in Kaposi's sarcoma. |
- DNA non-enveloped viruses
- -Parvovirus B19 (Aplastic anemia and effect on erythrocytes)
- -Carcinogenesis in other DNA non-enveloped (human papilloma viruses, polyoma viruses and adenoviruses)
|
- RNA enveloped viruses: Influenza A virus
- -General properties of influenza viruses, -Genetic (mutations)
- -Pathology of H1N1 infection
- -New trends in H1N1 chemotherapy and vaccination
|
- Pathology and pathogenesis in
- -Measles and SSPE (subacutesclerosingpanencephalitis)
- -Mumps virus complications and male sterility
- -RSV bronchiolitis in children under 2 years of age
- -Rotavirus gastroenteritis in children.
|
- Adinoviruses
|
- HEPATITIS VIRUSES
-Properties of hepatitis viruses (HBV and HCV genotypes classification)
-Chronic hepatitis B and C viruses (pathology)
-Role of HBV and HCV in hepatocellular carcinoma |
- Human Immunodeficiency virus HIV
-General properties and clinical manifestations
-Immuno-pathology and how HIV evades host immune system
-New trends in HIV chemotherapy and vaccination |
- Reo, Corona virus, SARS
|
- Mechanisms of viral carcinogenesis in
-DNA viruses
- -RNA viruses
|
- Viruses of Bacteria (Bacteriophages)
|
Credits: 3 credit = (2 hr theory lectures+ 2 hr practical)/week
Practical Sessions:
- Introduction to Practical Virology
|
- Methods of diagnosis of viral infections
|
- Preparation of tissue culture, Clinical specimens collection and handling:
|
- Inoculation of clinical sample in tissue culture, in embryo-egg, and in lab. Animals.
|
- ImmunoElecton Microscopy (IEM).
|
- Immunocytochemistry (ICC).
- Immunohistochemistry (IHC).
|
- In situ hybridization (ISH).
|
- Polymerase chain reaction (PCR) Extraction of N.A.
|
- Polymerase chain reaction (PCR)
|
- Indirect Methods: Serology.
|
- Clinical Applications.
|
- Clinical Applications.
|
Medical Parasitology
Second Course:
Credits: 3 credit = (2 hr theory lectures+ 2 hr practical)/week
General Parasitology
- Definition, Introduction to parasite, Host- parasite relationships ,Source of infection Mode of infection ,Attributes of pathogenic parasites,Attributes of host that resist infection,Direct physical & chemical changes,Tissue damage by immunological mechanism,Laboratorydiagnosis Classification of parasite
|
- Introduction to helminthes:Nematodes
- General features,Classification
- Enterobiusvermicularis ,Trichuristrichiura ,Ascarislumbricoides ,Strongyloidesstercoralis
|
- Hook worm :Visceral larva migrans ,Cutaneous larvae migrans
- Toxocaracanis,Dracunculusmedinensis
- Angiostrongyluscantonensis ,Gnathostoma spp.
- Capillaria spp.,Trichinellaspiralis
|
- Trematodes :General features ,Classification
- Schistasomiasis,Fasciola hepatica, F. buski
- Paragonimuswesermani.
|
- Introduction to Protozoa ,General features
- Classification
|
- Amoeba:Entamoebahistolytica
- Non-pathogenic amebae :
- E. dispar,E. hartmanni ,E. coli,E. gingivalis ,Endolimax nana, Ioddamoebabuetschlii
|
- Opportunistic amoeba :
- N aegleriafowleri ,Acanthamoeba spp.
|
- Flagellates (Intestinal,oral & genital flagellates ):Giardia lamblia,Trichomonastenax,T. hominis.
|
- Flagellates (Intestinal, oral & genital flagellates):
- T.vaginalis,Chilomasticmesnili,Dientamoebafragilis
|
- Blood & tissue flagellates :
- Visceral leishmaniasis,Cutaneous leishmaniasis , Mucocutaneousleishmaniasis
|
- Blood & tissue flagellates :
- Trypanosomagambiense ,T. rhodesiense, T. cruzi
|
- Sporozoa :Malaria Parasite ,Babesia.
|
- Sporozoa :Toxoplasma gondii ,Isospora belli , Cryptosporidium parvum, Sarcocystis,Blastocystishominis,Pnemocystisjirovecii
- Ciliophora :Balantidium coli
|
- Medical Entomology
|
Practical Sessions:
- Introduction,faecal examination
|
- Enterobius,Trichuris.Strongyloides, Ascaris
|
- Hookworms
|
- Echinococcus spp.
|
- Trichinella , Filaria,larvamigrans,
|
- Schistosoma
|
- Cultivation of helminthes Preservation of helminthes.
|
- Blood film and stool examination
|
- Introduction, faecal examination, Entamoeba. Other amoebae
|
- Flagellates, ciliates
|
- Culture methods used in Protozoology.
|
- Leishmania, Trypanosoma.
- Malaria (Plasmodium)
|
- Preservation of protozoa
|
- Animal inoculation as a method of diagnosis. Staining method.
|
- Medical Insects
|
Medical Mycology
Second Course:
Credits: 3 credit = (2 hr theory lectures+ 2 hr practical)/week
- Superficial Mycosis: Dermatophytosis, Keratomycosis, Malassezia spp., TineaNigra, &Piedra.
|
- Subcutaneous mycosis:Lobomycosis, Mycetoma, Sporotrichosis, &Chromoblastomycosis.
|
- Deep Mycosis: Aspergillosis, Blastomycosis.
|
- Candidiasis
|
- Coccidoidomycosis, Cryptococcosis
|
- Histoplasmosis&Pneumocystosis
|
- Penicillosis, &Protothecosis
|
- Fungal infection among AIDS patients.
|
- Antimycotic therapy
|
- Mycotoxicosis
|
- Lab. Dx. Of fungal infection
|
- Topics presented by students
|
Practical Sessions
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
- Data show of theoretical related fungal infection
|
Molecular Biology Curriculum for PhD students
Credits: 2 units: 2 theory lectures /week
1. Introduction
-DNA replication
-Mechanisms of replication
-Telomeres and telomerase |
2. Gene expression introduction:
- Mechanisms of gene expression
- Gene expression control for eukaryotics and prokaryotics
- Post-translational proteins folding
- Operons |
3. Microbial genetics introduction
- Concept ofallelic variation
- The in-lab auxotrophic and prototrophic selection of mutant cells
-Transformation – Conjugation-Transduction
-Chromosomal mapping in bacteria and in bacteriophages |
4. Molecular biology applications and tests (introduction)
5. Gel electrophoresis – RFLP-PFGE
6. Restriction endonucleases
7. Hybridization techniques |
8. Microarrays
9. Gene cloning |
10. PCR principles
11. Traditional PCR mechanisms
12. Traditional PCR optimization (full set of maneuvers)
13. Real-time PCR
- Quantification using real-time PCR
- Reverse transcriptase real-time PCR |
14.Mutation detection techniques |
15.DNA methylation and gene expression regulation |
16.Genomic imprinting |
17.Protein non-coding RNAs |
18.Genetic variation and DNA markers |
19.Introduction to Cytogenetic and Cytogenetic abnormalities |
20.Molecular biology of Mitochondrial DNA |
|
|